If you continue browsing the site, you agree to the use of cookies on this website. Itcan tolerate a wide range of soil types including saline, alkaline, sandy, heavy clay and rocky soils including oolitic limestone and. The success of the micropropagation procedure depends. Tissue culture is the in vitro aseptic culture of cells, tissues, organs or whole plant. Micropropagation of facifrora edulis slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The following species are recognised by plants of the world online powo. An exclusive project report on plant tissue culture. However, the queensland herbarium currently considers. Pongamia pinnata, floral phenology, pod maturation, fruit colours, seed and seedling quality characters.
Mar, 2017 micropropagation of facifrora edulis slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Plant tissue culture micropropagation ppt by deepak chaudhari authorstream presentation. Leaves can be used for stock feed in times of drought. Capital budgeting and cash flow analysis was conducted for a potential pongamia plantation and crushing plant in queensland, australia. The process includes many stepsstock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimation, and growing on of liners. A study was conducted to assess the distribution and extent of morphometric diversity among the population across major pongamia growing agroecological zones of karnataka, andhra pradesh and tamil nadu. Successfully been grown and conserved by micropropagation because of. Micropropagation is a sophisticated technique for the rapid multiplication of plants. Visual object recognition for automatic micropropagation of.
New tree species for agroforestry and energy purposes. To achieve this, in vitro regeneration of bamboo plantlets is needed due to the long and irregular bamboo flowering cycle and scarcity of bamboo seeds. Pierre, commonly known as karanja or honge, is a mediumsized glabrous, leguminous tree species indigenous to indian subcontinent and also found in southeast asia and has successfully introduced in other parts of the world. This is particularly valuable in the case of difficultto. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Article pdf available in journal of pharmaceutical sciences and research 33. Micropropagation is the asexual propagation of plants from growing tissue be it whole. Pongamia pinnata is a legume plant which has great potential to be used as a biofuel feedstock. An effective protocol for plantlets regeneration in b.
Micropropagation definition, the propagation of plants from tissue cultures, often from single cells. Tissue culture serves as an alternative to vegetative propagation by conventional methods, such as by rooting of cuttings. The plumbing is accomplished with various flexible hoses and some hard sprinkler tubing. Abstractbibliographic data and the results of present domestic experiments show that paulownia species can be grown easily, have high biomass production, favourable energetic parameters and modest requirements as to site quality. The bud break was reported on ms medium supplemented with 1.
Micropropagation definition and meaning collins english. Nodal shoots were used as explants and surfacesterilized with 0. Presentation about microprogation in plant tissue culture created by. Pongamia is an out breeding species, thus seed populations exhibit high levels of. For annual seed yields ranging from 20 to 80 kg in shell per tree, the delivered cost of. Micropropagation of tuna opuntia ficus indica and effect. Its native range is uncertain, with conflicting information in the published literature. Micropropagation is a sophisticated technique for the rapid.
Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches. Nekl, assistant professor of biology cofaculty proposers. A complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Micropropagation from shoot tips of 20 to 30 days old aseptically germinated seedlings of c. Definition of micropropagation clonal propagation in vitro micro propagation is the production of whole plant from small section of plant such as a stem tip, node, meristem, embryo, or even a seed. Micropropagation definition of micropropagation at. Project report on the meaning of plant tissue culture project. A protocol for in wk propagation of this plant was standardized using seedling explants. Definition of micropropagation clonal propagation in vitro micropropagation is the production of whole plant from small section of plant such as a stem tip, node, meristem, embryo, or even a seed. A tissue culture technique for plant propagation in which tissue is taken from a plant and grown in a laboratory to produce plantlets that are. Introduction of disease free new cultivars is possible through micropropagation 6. Applications of plant tissue culture in crop improvement 5.
Micropropagation definition of micropropagation by the free. On testing auxins for their effect on shoot proliferation, only iaa together with bap showed an effect. Melhania is a genus of small shrubs or herbaceous plants. High demand for edible bamboo shoots of bambusa tulda and melocanna baccifera in many asian ethnic groups has led to the need for developing intensive bamboo farming. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. Virus free plant sub millimeter portion of shoottip stock plant virus free nodal cutting disadvantage of using small sized explant for micropropagation shoot tip explant low survival rate and slow initial growth. Micropropagation of pongamia pinnata through enhanced.
Analysis of genetic diversity in pongamiapongamia pinnata l. Technology and application and a great selection of related books, art and collectibles available now at. The peashaped flowers vary from pink to mauve and these are followed by indehiscent, brown, flattish woody pods about 2 cm long. Insects galls commonly present appear like small brownish peas. Thus, seed populations exhibit high levels of heterogeneity. Micropropagation of plants is done by cutting juvenile plants and placing them into special containerboxes with nutrientsolution where the pieces can grow up and be cut again several times.
Pongamia millettia pinnata, formerly known as pongamia pinnata, is a treeshrub with a broad distribution from india, through central and southeastern asia, indonesia and into northern australia. Multiplication of genetically identical copies of a cultivar by asexual reproduction using tissue culture techniques iv requirements. Murashige and skoog ms medium was used to establish the cultures of r. Pongamia millettia pinnata has been widely studied as a potential feedstock for biodiesel fuel, though little is known about its feasibility at a commercial level. Plant tissue culture micropropagation ppt by deepak chaudhari. Since then, the staff has been hard at work trying to save this living connection to john muir. It is also used to provide a sufficient number of plantlets for planting from a stock plant. The potential of this oil as a substitute for diesel srinivasa 1997. Introduction biodiesel is an excellent alternative for petroleum diesel substitution. It is identified as a sustainable substitute for petroleum fuel. A tissue culture protocol was developed for an important medicinal plant rungia pectinata l.
Multiple shoots were induced on ms medium supplemented with bap. Approximately 400 cuttings from the original tree were sent to archangel ancient tree archive in april 20. Multiple shoot bud induction and plant regeneration. Pongamia pinnata,activated carbon,biodiesel,karanja,adsorbent. Pongamia pinnata is an important biofuelyielding tree species. Economics of oil production from pongamia millettia. Micropropagation of tuna opuntia ficus indica and effect of medium composition on proliferation and rooting akram ghaffari 1, tahereh hasanloo 1, mojtaba khayam nekouei 2 1department of molecular physiology, agricultural biotechnology research institute of iran abrii, karaj, iran. Pdf micropropagation of mature pongamia pinnata pierre.
Micropropagation enables growers to increase the production of plants that normally propagate very slowly such as narcissus and other bulbous crops. It has a great commercial potential due to the speed of propagation, the high plant quality, and the ability to produce diseasefree plants. Vegetative propagation of sterile hybrids can be used as parent plants for seed production. Experiences on propagation, planting and plant management by the use of specifically selected paulownia plants and selfdeveloped propagation method we managed to realize. It presents important characteristics as nontoxicity, biodegradability, high energy content and absence of sulfur. Usually meristem or buds without callus reduced somoclonal variation axillary branching. Visual object recognition for automatic micropropagation. Chapter2 micropropagation and large scale culture of c. Analysis of genetic diversity in pongamiapongamia pinnata. Micropropagation is the art and science of plant multiplication in vitro. Principles of tissue cult ure and micropropagation.
Archangel ancient tree archive has successfully cloned john muirs beloved giant sequoia. Micropropagation definition of micropropagation by the. Explants carrying performed vegetative bud suitable for enhanced axillary branching. The plumbing is accomplished with various flexible hoses and some hard sprin. On testing auxins for their effect on shoot proliferation, only iaa. Pongamia pinnata is a fastgrowing, mediumsized, evergreen or briefly deciduous, glabrous shrub or tree with a broad crown of spreading or drooping branches 303 title. Micropropagation powerpoint slide set with photos note. Traditionally included in the family sterculiaceae, it is included in the expanded malvaceae in the apg and most subsequent systematics.
Pelagia research library advances in applied science research, 2014, 51. Project report on plant tissue culture biology discussion. Pongamia pinnata pierre is a tree legume, having potential in production of raw material for biodiesel. Multiple shoots were induced in vitro from nodal segments through forced axillary branching. Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot multiplication 93 8 fold multiplication rate per month 9preexisting meristems are least susceptible to genetic changes micropropagation stages stage 0. Oct 16, 2007 a complete protocol is presented for the first time for the micropropagation of pongamia pinnata, a biofuel tree, using cotyledonary nodes derived from axenic seedlings. Micro propagation free download as powerpoint presentation. Pierre leguminaceae, subfamily papilionoideae is a medium sized tree commonly called as karanja that generally attains a height of about 1020 m and a trunk diameter of more than 50 cm pongamia. Pierrea native indian biodiesel tree from cotyledonary node. Micropropagation and regeneration of herbaceous perennials native to the eastern united states.
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